Part:BBa_K2814025
TP901-1 Integrase
TP901-1 integrase comes from TP901-1 phage of Lactococcus lactis subsp. Cremoris. It can bind to specific attB/P sites to catalyze DNA recombination. The TP901-1 phage integrates its genome into the bacterial genome using the integrase. By constructing the attB/P sites in different directions, TP901-1 can catalyze the recombination of DNA between their sites, leading to inversion when attB/P are in opposite directions and excision when attB/P are in the same directions. TP901-1 is widely used to construct combinational logic gates and performs well in changing DNA sequence. TP901-1 is one of the recombinases with outstanding performance. In the existence of recombinase TP901-1, different orientation of attB and attP allows the sequence to be flipped, excised, or inserted between recognition sites, which makes it useful for gene editing. In our project, the TP901-1 integrase binds to a 59 bp attB (BBa_K2814013) sequence and 50 bp attP (BBa_K2814011) sequence. We have used the TP901-1 Integrase for the flipping in our Recombinase Based Incoherent Feed Forward Loops. The integrase forms the first node of our RIFFL circuit, with the secondary node (BBa_K2814016) and tertiary node ( BBa_K2814020)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1453
- 1000COMPATIBLE WITH RFC[1000]
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